ytiB [2018-02-12T18:26:45.000Z]

similar to carbonic anhydrase

locus: BSU30690

pI: 6.17

mw: 20.97 kDa

protein length: 187 aa

gene length: 561 bp

function: unknown

product: unknown

essential: no

ec: 4.2.1.1

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,138,321 → 3,138,884

The protein

Catalyzed reaction/ biological activity

• H₂CO₃ = CO₂ H₂O (according to Swiss-Prot)

Protein family

• beta-class carbonic anhydrase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|5D96DF2F32050BBCEC3811843398FE84DE88F6DF|YbcF], [protein|E00889289B07BE78CC0C8E0962A55C3B14871E00|YvdA] (57,8%)

Structure

• [PDB|1G5C] (from Methanobacterium Thermoautotrophicum 34% indentity)

Expression and Regulation

Operons

• genes: [gene|F942C0F4618FACEF35ACA629355BAC288556E7B6|ytiB]-[gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

Biological materials

Mutant

• MGNA-A293 (ytiB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/293 NBRP B. subtilis, Japan]
• BKE30690 (Δ[gene|F942C0F4618FACEF35ACA629355BAC288556E7B6|ytiB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCGTTGTCCCTTTCT, downstream forward: _UP4_CTTATCGTAAACGGTTATAA
• BKK30690 (Δ[gene|F942C0F4618FACEF35ACA629355BAC288556E7B6|ytiB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCGTTGTCCCTTTCT, downstream forward: _UP4_CTTATCGTAAACGGTTATAA

References

• 26577401